Inhibition:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Expressing:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Transfection:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Cell Counting:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Flow Cytometry:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Binding Assay:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Control:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Small Interfering RNA:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Over Expression:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Migration:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
Negative Control:Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer
Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observation and mycoplasma testing.
Article Title: Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancer
Article Snippet: Cell culture and transfection.Cell culture and transfection.. Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB‐35) and HeLa (cat. no. CRM‐CCL‐2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP‐5091; Beijing, China).. Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.Although short tandem repeat analysis was not performed, the HaCaT cells were purchased from a certified national cell bank with traceable origins and their authenticity was confirmed by morphological observa‐ tion and mycoplasma testing.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultiva
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ). .. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 TM , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 TM ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 TM ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA). .. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1.
Article Snippet: The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection (4).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35TM, American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31TM; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614TM; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37 ̊C with 95% humidity and 5% CO2.
Article Title: Upregulation of lncRNA GATA6-AS suppresses the migration and invasion of cervical squamous cell carcinoma by downregulating MTK-1
Article Snippet: Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).Cell lines, cell culture and transfection The occurrence of cervical squamous cell carcinoma is significantly associated with HPV infection ( 4 ).. Therefore, the present study employed the two human cervical squamous cell carcinoma cell lines SiHa [HPV-positive; cat. no. HTB-35 ™ , American Type Culture Collection (ATCC), Manassas, VA, USA] and C33A (HPV-negative; cat. no. HTB-31 ™ ; ATCC), and the two normal cervical cell line; Ect1/E6E7 (HPV-positive; cat. no. CRL 2614 ™ ; ATCC) and HCvEpC (HPV-negative; cat. no. 233-75a; Cell Applications, San Diego, CA, USA).. Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .Eagle's Minimum Essential Medium supplemented with 10% FBS (Sangon, Shanghai, China) was used to cultivate the cells at 37°C with 95% humidity and 5% CO 2 .
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